A master’s thesis was discussed at the College of Health and Medical Technology at the Southern Technical University on the spread of cyclomululin and genotoxin-producing genes in Escherichia coli strains isolated from urinary tract infections and bladder cancer in southern Iraq. The thesis submitted by student Sarah Ali Nimah aims to evaluate the spread of positive Escherichia coli bacteria among patients with urinary tract and bladder cancer in Iraq, and to identify and classify Escherichia coli isolates, in addition to detecting the cytotoxic necrosis factor. The thesis recommended conducting an expanded study of the effect of these toxins by following up on the condition of the same patients. Those infected with bacteria that carry genes that produce genotoxins, in addition to continuing to conduct antibiotic susceptibility testing for Escherichia coli that causes urinary tract infection to determine its sensitivity to it and thus the possibility of using appropriate treatments due to the increase in resistant strains.